2nd Workshop on TritiGen COST action FA0604: Triticeae genomics for the advancement of essential European crops

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Monday, September 22

8:00Shuttle bus service from hotel Gergana to Flamingo Grand Hotel
8:00-8:45Registration and poster set up
8:45-9:00 Welcome

Session 1: WG1: Building of consensus and high-density maps. Includes development and use of COS markers from model genomes

Chairs: Karl Schmid and Hikmet Budak

09:00 Neslihan Ergen (Turkey)
Sequencing 12,000 EST from Triticum dicoccoides following exposure to drought stress shock
09:20 Sophie Paillard (France)
Components of adult plant resistance to Puccinia striiformis in the bread wheat Apache.
09:40 Edita Gregova (Slovakia)
Diversity of HMW-glutenin alleles in European wheat landraces.
10:00 Sabina Chebotar (France/Ukraine)
Variability analysis of 165 microsatellite loci of homoeologous group 3 chromosomes of bread wheat (T. aestivum L.).
10:20-11:10 Coffee Break and poster viewing
11:10 Karl Schmid (Germany)
GABI-GENOBAR: A genome wide approach to associate genetic diversity to agronomically important traits in barley
11:30 Yehoshua Saranga (Istrael)
Genomics dissection of grain protein and mineral concentrations in wild and domesticated wheat species.
11:50 Ruslan Kalendar (Finland)
Use of retrotransposon markers to analyse genetic diversity of wild emmer wheat (Triticum dicoccoides).

12:10 WG1 Discussion on proposals for cooperation

12:10 Proposal by Andras Balint (Hungary)
Discussion panel: Andras Balint, Karl Schmid and Hikmet Budak
Co-operation proposal between TritiGen-members: Development of a marker assisted selection system for breeding cereals with better nutritional value using association mapping.
12:30-13:30 Lunch in Essentials restaurant, Flamingo Grand Hotel

Session 2: WG2: Update and discussion on strategies for building contigs from BACs in barley and wheat. Application of model genomes for this purpose.

Chairs: Pierre Sourdille and Nils Stein

13:30 Delfina Barabaschi
Physical mapping of the barley Fr-H2 (Frost resistance-H2) locus
13:50 Elitsur Yaniv (Israel)
Towards positional cloning of the stripe rust resistance gene Yr15 derived from wild emmer wheat
14:10 Jérome Salse (France)
Identification and Characterization of COS-SSCP Markers for Fine Mapping in Cereals
14:30 Daniela Schulte (Germany)
Current status in developing a genome-wide physical map in barley (Hordeum vulgare L.)
14:50 Etienne Paux (France)
A glimpse into the impossible: chromosome-based physical mapping of the giant bread wheat genome
15:10-15:30 Coffee Break and poster viewing
15:30 Federica Cattonaro (Italy)
Progress in high information content fluorescent for physical mapping of what chromosomes
15:50 Helene Berges (France)
How to preserve, maintain and exploit wheat and barley genomic resources: from the BAC library to the 3-Dimensional pools
16:10-16:50 General discussion

Session 3: WG 2A: Databases and web-based tools for annotation and genome comparisons.

Chair: David Marshall `
16:50 Frederic Choulet (France)
Sequence analyses of Megabase-sized contigs of the wheat genome
17:10Heidrun Gundlach (Germany)
How to tackle the repeat Junk of grass genomes
17:30-17:50 Tamar Krugman (Israel)
Global gene expression patterns of drought resistance vs. drought susceptible wild emmer wheat genotypes
18:00-19:30 Wine testing and poster viewing
20:00 Buffet dinner (hotel Gergana restaurant)

Tuesday, September 23

Session 4: WG3: Novel uses of genomics technologies for identification of candidate genes and positional cloning in Triticeae

Chairs: Nils Rostoks and Hilde-Gunn Opsahl- Sorteberg

08:30 Shuttle bus service from Hotel Gergana to Flamingo Grand Hotel
09:00 Eva Bauer (Germany)
The GABI RYE Genomics Network
09:20 Gábor Galiba (Hungary)
Interaction between vernalization and frost tolerace in cereals
09:40 Marco Maccaferri (Italy)
The widely exploited-durable leaf rust resistance from durum wheat Creso maps in the distal region of chromosome arm 7BL
10:00 María-Pilar Vallés (Spain)
Transcriptome Comparison of Three Barley Lines Reveals Genes Involved in microspore embryogenesis
10:20-10:50 Coffee Break and poster viewing
10:50 Agnieszka Janiak (Poland)
Genetic and molecular dissection of root hair development in barley
11:10 János Györgyey (Hungary)
Use of transcriptional profiling to find candidate genes involved in the drought adaptation of the root system of cereals
11:30 Nils Rostoks (Latvia)
Candidate gene identification and analysis for characterization of barley necrotic mutants
11:50 David Marshall (UK)
Designing a 44k barley Agilent microarray for barley
12:10-13:00 Discussion: New microarray technologies for wheat and barley
13:00-14:00 Lunch in Essentials restaurant, Flamingo Grand Hotel

Session 5: WG 4: Transformation resources and progress in Europe

Chairs: Sřren Rasmussen and Patrizia Galeffi

14:00 Huw D. Jones (UK)
Temporal and Spatial Targeting of Transgene Expression
Current status of cereal transformation in the EU
14:25 Jochen Kumlehn (Germany)
Genetic engineering in cereals: Current technologies for the elucidation of gene function
14:45 Paul Christou (Spain)
Combinatorial genetic transformation for the engineering of complex biosynthetic pathways in plants
14:55 Merete Albrechtsen (Denmark)
On the use of Barley stripe mosaic virus for gene silencing in barley
15:15 Pierre Barret(France)
Simple and Stable Transgene Integration through Biolistic Transformation of Wheat with Dephosphorylated Gene Cassettes
15:35-16:00 Coffee break and poster viewing
16:00 Kiara Merzaros (Hungary)
Genetic transformation of immature embryos in spring and winter wheat via particle bombardment and Agrobacterium co-cultivation
16:20 Mariann Rakszegi (Hungary)
Transformation experiments to support quality oriented wheat breeding
16:40 Michela Janni (Italy)
Wheat transformation with polygalacuronase or pectin methyl esterase inhibitors to improve plant defence
17:00 Inger B. Holme (Denmark)
Cisgenic barley for animal feed
17:20 Aariana Latini (Italy)
Study of the function of the TdDRF1 proteins: transient expression of the three related transcripts in Nocotiana plants
17:40 Eva Stoger (Austria)
Targeting recombinant proteins in the cereal grain
18:00 Otto Torjek (Hungary)
EcoTILLING analysis of candidate genes for drought tolerance in barley
18:20-18:30General discussion

Session 6: WG 2A: Databases and web-based tools for identification of candidate genes, positional cloning and function prediction in Triticeae

Chair: Thomas Wicker

18:30 Ali Banaei (Germany)
Analysis of relationship between intra-species hybridization and level of DNA/histone modifications in Arabidopsis thaliana
18:50 Abraham Korol (Israel)
Improving the efficiency of contig assembly for physical mapping based on restriction fingerprinting
19:10 Catherine Feuillet (France)
Administrative session (restricted to participants of the TriticeaeGenome project)
19:20 Poster dismounting
20:30 Workshop Official Dinner in restaurant Blue sky, Hotel Dobrudja, 17th floor

Wednesday, September 24

08:30 Shuttle bus service from hotel Gergana to Flamingo Grand Hotel

Session 7: Joint session (5 WGs)

Chairs: Alan Schulman and Catherine Feuillet

09:00Reports from the WG coordinators of the discussions and perspectives for each WG.
10:30 Catherine Feuillet: Report on the status of project applications to EU calls (FP7 and ERA-PG).
10:45 Concluding remarks from the Action's Chairs (Alan Schulman and Catherine Feuillet)
11:00-11:30Coffee break
11:30-13:30Management Committee meeting (closed)
13:30-14:30Lunch in Essentials restaurant, Flamingo Grand Hotel
14:30Transfer to Plant GEM

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